FIG. 3. .

Foxp3 mRNA is not detected in adult mouse pituitary or hypothalamus. Foxp3 expression levels were measured in pituitary (A, B) and hypothalamus (A, C) from adult C57BL/6J male mice. Foxp3 expression in thymus was used as a positive control for our Foxp3 assay (A). Foxl2 and Gnrh expression was used as a positive control for the integrity of the RNA from pituitary (B) and hypothalamus (C), respectively. Products from PCR (A) and real-time PCR (B, C) were visualized by agarose gel electrophoresis.