| pCR®2.1-TOPO |
PCR cloning vector |
Invitrogen |
| pMAL-p2x |
Expression vector with an N-terminal maltose binding protein tag |
New England Biolabs |
| pET23a |
Expression vector with a C-terminal 6-His tag |
Novagen |
| pTV146 |
bb0685 cloned into pCR2.1 for protein expression |
This study |
| pTV147 |
bb0685 cloned into pMAL-p2x |
This study |
| pTV118 |
bb0686 cloned into pCR2.1 |
This study |
| pTV125 |
bb0686 cloned into pET23a |
This study |
| pTV119 |
bb0687 cloned into pCR2.1 |
This study |
| pTV101 |
bb0687 cloned into pET23a |
This study |
| pTV120 |
bb0688 cloned into pCR2.1 |
This study |
| pTV121 |
bb0688 cloned into pET23a |
This study |
| pTV131 |
bb0683 cloned into pET23a |
This study |
| pET45b/lmhmgr
|
Lmhmgr cloned into pET45b |
[23]
|
| pYL03 |
bb0589 cloned into pET23a |
This study |
| pYE04 |
bb0622 cloned into pET23a |
This study |
| pCR2.1/Pflac
|
Pflac cloned into pCR2.1 |
Unpublished data, D. Scott Samuels |
| pTV142 |
Pflac cloned into pCR2.1 with KpnI and NheI restriction sites |
This study |
| pTV145 |
bb0685 cloned into pCR2.1 with NheI and EcoRV/KpnI restriction sites |
This study |
| pTV146 |
Pflac/bb0685 cloned into pCR2.1 with flanking KpnI sites |
This study |
| pBVS2/lacI
|
Borrelial shuttle vector with kanr and lacI under the control of PflgB
|
[55], [56]
|
| pTR1 |
pBVS2/lacI with Pflac/bb0685
|
This study |
