Figure 5. rSdrE-bound fH retains cofactor activity for factor-I mediated cleavage of C3b.

rSdrE was immobilized to a microtiter plate and incubated with purified fH; anti-fH and ovalbumin (OVA) were used as controls. After washing, C3b and factor I were added. Well contents were assessed via anti-C3 Western blot for C3b and iC3b. A, Samples with or without 2 µg fH. B, rSdrE with varied amounts of purified fH. Representative Western blots of at least three independent experiments are shown.