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. 2012 May 31;7(5):e38407. doi: 10.1371/journal.pone.0038407

Figure 6. Serum fH binds to SdrE-expressing L. lactis in a dose-dependent manner.

Figure 6

A, L. lactis isogenic mutants were immobilized to a PVDF membrane and overlaid with 5% HI-serum; fH binding was quantitated using a serum fH standard curve and optical densitometry; *p = 0.02. B, L. lactis isogenic mutants were incubated with various concentrations of HI-serum for 1 hr; bound proteins were extracted with 2% SDS and analyzed by fH ELISA using chicken antibodies specific for human fH; p = 0.005, as a group. C, L. lactis were incubated with 20% HI-serum; bound proteins were extracted with 2% SDS and analyzed by anti-fH Western blot. Data represent the mean of at least three independent experiments ± SEM.