Skip to main content
. 2012 May 31;7(5):e38373. doi: 10.1371/journal.pone.0038373

Figure 1. LPS induces COX-2 in IEC-6 cells.

Figure 1

A) IEC-6 cells were treated with the indicated concentrations of LPS for 24 hours. B) IEC-6 cells were treated with 2 µg/mL LPS for the indicated time. C) IEC-6 cells were treated with LPS (2 µg/mL) for the indicated times. D) Cells were treated with LPS (2 µg/mL), EGF (10 ng/mL), or co-treated with LPS and EGF for 24 hours. Protein expression was determined by Western blot analysis and densitometry. Treatment with LPS did not induce COX-1 expression in IEC-6 cells. In contrast, treatments with LPS, EGF, or both significantly increased COX-2 expression compared to control (p<0.001,  = 0.05, and <0.001 respectively). Cells treated with LPS and EGF had significantly greater COX-2 expression than by either EGF (p = 0.002) or LPS (p = 0.006) alone. Single asterisks indicate significant differences from control. Double asterisks indicate significant differences between two bracketed conditions.