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. 2012 May 31;7(5):e38373. doi: 10.1371/journal.pone.0038373

Figure 2. Transactivation of EGFR by LPS induces COX-2 expression in IEC-6 cells in an MMP- and p38-dependent fashion.

Figure 2

A) IEC-6 cells were treated with LPS (2 µg/mL) for the indicated time or with EGF (10 ng/mL) for 5 minutes. EGFR immunoprecipitates were assayed for P-EGFR by Western blot analysis. B) Western blot analysis of cells treated with LPS (2 µg/mL) or EGF (10 ng/mL) for 24 hours in the presence or absence of the EGFR kinase inhibitor AG1478 (1 µM). C) IEC-6 cells were stimulated with LPS (2 µg/mL) for 24 hours in the presence or absence of the Src family kinase inhibitor CGP77675 (2 µM) or the MMP inhibitor GM6001 (50 µM). D) IEC-6 cells were stimulated with LPS (2 µg/mL) for 15 minutes in the presence or absence of CGP77675 (2 µM), GM6001 (50 µM), or p38 MAPK inhibitor SB202190 (10 µM). Single asterisks indicate significant differences from control. Double asterisks indicate significant differences between two bracketed conditions.