Skip to main content
. 2012 May 31;7(5):e38344. doi: 10.1371/journal.pone.0038344

Figure 1. Single FV and VF events detected by FRET between Cy3-F and Cy5-V during translation of mRNA-1 (FV: A, B) and mRNA-2 (VF: C, D).

Figure 1

In the cartoons (A, C), fluorescent labels are shown as filled colored circles: Cy3 is green, Cy5 is red. Black and blue letters represent the tRNAs and codon triplets, respectively, for a given amino acid, using the standard single-letter abbreviations. For ease of presentation, all three tRNA sites are shown to be occupied during the entire event, but this need not be the case [13]. The excitation wavelength was alternated (ALEX) between 532 nm and 640 nm every other image. The traces in (B, D) show Cy3 fluorescence (green trace, 585 nm detection) and sensitized emission of Cy5 (FRET, blue trace, 680 nm detection), both under 532 nm excitation, and Cy5 fluorescence (red ALEX trace, 680 nm detection) under direct 640 nm excitation.