Table 1. mRNAs used in this study.

mRNA	Coded peptide sequences	mRNA sequence
1	M RFV (YFV)6 RFV (YFV)6	GGG AAU UCG AAA UAG AAG UCU UCU UUU UGG A AAA AUU UAA AAG UUA AUA AGG AUA CAU ACU AUG CGU UUU GUG UAU UUU GUG UAU UUU GUG UAU UUU GUG UAU UUU GUG UAU UUU GUG UAU UUU GUG CGU UUU GUG UAU UUU GUG UAU UUU GUG UAU UUU GUG UAU UUU GUG UAU UUU GUG UAA CGC GUC UGC AGG CAU GCA AGC UAA AAA AAA AAA AAA AAA AAA AAA AAA GCU
2	M RVF (YVF)6 RVF (YVF)6	GGA AAA AUU UAA AAG UUA AUA AGG AUA CAU ACU AUG CGU GUG UUC UAU GUG UUC UAU GUG UUC UAU GUG UUC UAU GUG UUC UAU GUG UUC UAU GUG UUC CGU GUG UUC UAU GUG UUC UAU GUG UUC UAU GUG UUC UAU GUG UUC UAU GUG UUC UAU GUG UUC UAA CGC GUC UGC AGG CAU GCA AGC UAA AAA AAA AAA AAA AAA AAA AAA AGG ATC CCT AGC ATA ACC CCT TGG GGC CTC TAA ACG GGT CTT GAG GGG TTT TTT GA
3	MRFVRFVRF	GGG AAU UCA AAA AUU UAA AAG UUA AUA AGG AUA CAU ACU AUG CGU UUC GUG CGU UUC GUG CGU UUC
4	MFRVFRVFR	GGG AAU UCA AAA AUU UAA AAG UUA AUA AGG AUA CAU ACU AUG UUC CGU GUG UUC CGU GUG UUC CGU
5	M (VE)6 (RFV)2 RFK (RFV)2 RFM	AAU UCA AAA AUU UAA AAG UUA AUA AGG AUA CAU ACU AUG GUU GAA GUU GAA GUU GAA GUU GAA GUU GAA GUU GAA CGU UUU GUU CGU UUU GUU CGU UUU AAA CGU UUU GUU CGU UUU GUU CGU UUU AUG UUC UUC UUC UUC UUU UUU UUU GUCUUC CUG CAG UUU UUU UUU UUU UUU UUU UUU UUU UUU A
6	M RFV YVF (YFVYVF)3 RFV YVF (YFVYVF)3	GGA AAA AUU UAA AAG UUA AUA AGG AUA CAU ACU AUG CGU UUU GUG UAU GUG UUC UAU UUU GUG UAU GUG UUC UAU UUU GUG UAU GUG UUC UAU UUU GUG UAU GUG UUC CGU UUU GUG UAU GUG UUC UAU UUU GUG UAU GUG UUC UAU UUU GUG UAU GUG UUC UAU UUU GUG UAU GUG UUC UAA CGC GUC UGC AGG CAU GCA AGC UAA AAA AAA AAA AAA AAA AAA AAA AGG ATC CCT AGC ATA ACC CCT TGG GGC CTC TAA ACG GGT CTT GAG GGG TTT TTT GA

The bold sequence for each mRNA is the translation start site, initiator fMet. Sequences are listed from 5′ to 3′ ends. The biotin tag for tethering to the microscope slide surface was at the 3′ end for mRNAs -1, -2, -5, and -6, and at the 5′ end for mRNAs -3 and -4.