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. 2012 May 31;7(5):e38280. doi: 10.1371/journal.pone.0038280

Figure 3. Silencing of endogenous G0S2 expression in BM cells increases blood chimerism upon transplantation.

Figure 3

Expression of the endogenous G0S2 gene was silenced in BM cells using two G0S2-specific pSIREN-shRNA retroviruses (Sh1, Sh2). Luciferase pSIREN-shRNA (Luc) was used as a control. (A) Knockdown efficiency was determined by quantitative real-time PCR in transduced BM cells (n = 4). (B) Sixteen weeks after transplantation, blood chimerism was measured by flow cytometry (n = 3–4). (C) The contribution of donor-derived cells to the Gr-1+ CD11b+ and CD3+ T cell populations was analyzed at different times after the transplant (n = 3–4). *, P<0.05, **, P<0.01, and ***, P<0.001 (two-tailed Student's t-test).