Figure 6. Inhibition of cell proliferation by G0S2 correlates with cytosolic retention of nucleolin.

(A) The effects of G0S2 deletion mutants on the proliferation of NIH-3T3 cells were examined by propidium iodine staining and flow cytometric analysis (n = 3). G0S2-wt was compared to G0S2 mutants and empty vector (Ctrl). *, P<0.05 (two-tailed Student's t-test). (B) Subcellular localization of endogenous nucleolin was determined by immunofluorescence in NIH3T3 cells transduced with the retrovirus containing full-length G0S2 or the deletion mutants described in Figure 5. The inset shows a higher magnification of a cell presenting nucleolar versus perinuclear localization. The data represent two independent experiments.