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. 2012 May 31;7(5):e36846. doi: 10.1371/journal.pone.0036846

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Bisulfite sequence analysis of DNA methylation of the γ-globin 5′ promoter region in erythroid progenitors grown in methylcellulose media, liquid media, or in co-cultures containing the AFT024 stromal cell line. Five CpG sites numbered relative to the transcription start site were analyzed. Each row represents the sequence analysis of a single amplicon cloned within the pCR4 vector. Unmethylated sites (green), methylated site (red), polymorophic sites where no CpG is present (yellow). B. Effect of decitabine on globin chain synthesis (γ/γ+β) during erythroid differentiation of baboon BM progenitors in AFT024 co-cultures. Decitabine (5×10⁻⁷ M) was added to cultures on d7. Globin chain synthesis was analyzed on d11 and d14. C. Globin chain synthesis (γ/γ+β) during erythroid differentiation of human cord blood progenitors in AFT024 co-cultures.