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. 2012 May 31;7(5):e37304. doi: 10.1371/journal.pone.0037304

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Hagemann et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) p62 immunohistochemistry in hippocampus from Gfap^+/R236H/Nrf2^+/+ mice shows astrocytic cells that are not apparent in Gfap^+/+ mice. Gfap^+/R236H/Nrf2^−/− mice show similarly stained cells (3 mos of age). (B) Confocal microscopy with immunofluorescence for p62 (green) shows colocalization with Gfap aggregates (red) in both Nrf2^+/+ and Nrf2^−/− Gfap^+/R236H mice (8 wks of age). (C) Western blot analysis of hippocampal protein shows increased p62 in Gfap mutant mice compared to wild-type, with Gfap^+/R236H/Nrf2^−/− mice having levels reduced to that of wild-type mice (8 wks of age, n≥4, t-test **p>0.01, comparing Gfap^+/R236H/Nrf2^+/+ with Gfap^+/R236H/Nrf2^−/−). Scale bar = 25 µm (shown in B, applies to both confocal images).