Skip to main content
. 2012 May 31;7(5):e37898. doi: 10.1371/journal.pone.0037898

Figure 2. Impact of salt intake on 11β-hydroxysteroid dehydrogenase type 2 expression in the colon of control and UNX rats.

Figure 2

A: mRNA expression in epithelial cells isolated from colon (real-time PCR). Values were normalized with endogenous β-actin mRNA expression. Results were quantified relative to the mean of the sham-operated, normal salt group. B: Western blots showing protein in epithelial cells isolated from colon. Lanes 1–7: Rat colon samples; Lane 1*: Sample 1 of UNX high NaCl group was loaded on each gel as a standard for comparison between blots; MW: molecular weight. C: Amount of proteins relative to control rats (sham-operated, normal salt). Values were adjusted with the colon protein sample on lane 1* and normalized with the protein expression of actin. The horizontal line is the median (n = 7). ★★, P≤0.01; ★★★, P≤0.001 indicate the effect of salt analyzed by two-way ANOVA (curly brackets; NS: normal salt; HS: high salt).