Figure 3. Regulation of general translation throughout the Leishmania infantum life cycle.

Promastigote to amastigote differentiation (A). Polysome profile analysis of L. infantum promastigotes (Pro) and axenic amastigotes (Ama) grown in MAA 20 medium at 37°C and pH 5.5 for 4 days in average (1^st passage). L. infantum differentiates into amastigote-like forms after ∼4 days of growth under these conditions as illustrated in (A). Amastigote to promastigote differentiation (B). Polysome profile analysis of L. infantum axenic amastigotes transferred from the MAA 20 medium at 37°C and pH 5.5 to a MAA medium at 25°C and pH 7.3 for up to 8 hours. Data displayed here represent one of three separate experiments. (C) The effect of L. infantum amastigote to promastigote differentiation on global protein synthesis as estimated by the incorporation levels of [³⁵S]-Met into the Leishmania proteins (as described in Figure 2). Results are the mean of four independent experiments.