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. 2012 May 31;7(5):e35085. doi: 10.1371/journal.pone.0035085

Figure 5. Translation of Leishmania housekeeping genes under stress.

Figure 5

(A–B) Northern blot hybridization of L. infantum RNA samples isolated from sucrose gradient fractions (F: free mRNPs; M: monosomes; L: light polysomes; H: heavy polysomes) to determine the association of the constitutively expressed alpha-tubulin mRNA with translating polysomes in the absence of stress (no stress) or under temperature (T°) or acidic pH stresses (A) and or in the presence of the endoplasmic reticulum (ER)-inducing agents thapsigargin (Tg) or tunicamycin (TN) (B, upper panels). The ethidium bromide-stained gels used for northern blot analysis served as a loading control (rRNA is indicated). Polysome profiling analysis of L. infantum promastigotes untreated (no stress) or treated with 0.4 µM of thapsigargin (Tg) for 4 hours (B, lower panels). Data shown here are representative of at least four independent experiments with similar results.