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. 2012 May 31;7(5):e38005. doi: 10.1371/journal.pone.0038005

Figure 7. TRPA1-medated insulin release and Ca2+ influx following knockdown of TRPA1 by siRNA.

Figure 7

a. RT-PCR after knockdown of TRPA1 by siRNA in control and mock-transfected cells. b. AITC (200 µM)-induced insulin release is significantly reduced after siRNA knockdown of TRPA1 in RIN cells (n=6, * p<0.05). c. AITC (200 µM) and MG (1 mM) induce increase in intracellular Ca2+ levels in mock-transfected RIN cells (upper panel), but not in siRNA treated cells (lower panel). Note that siRNA treated cells responded to KCl (50 mM) similar to that of mock-transfected cells.