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. 2012 May 31;7(5):e37630. doi: 10.1371/journal.pone.0037630

Figure 2. Functional complementation of the E. coli mutants impaired in iron release from heme.

Figure 2

E. coli strains FB8.27 pAM::hasR (pBAD24) (A), FB8.27 efeB::Kan yfeX::Cmp (pAM::hasR) (pBAD24) (B) and FB8.27 efeB::Kan yfeX::Cmp (pAM::hasR ), (pBAD24::hemS his) (C) were tested for the use of heme as an iron source on iron depleted medium M63 (Gly 0.4%, Ara 0.2%, Dip 70 µM, Spc, Amp). Growth around the wells containing 1 µM, 5 µM, 10 µM, or 50 µM Hb were performed as described in “Materials and Methods”. Growth around the wells was assessed by visible turbidity in the agar. These pictures were taken after 48 hours of growth at 37°C. Experiment was repeated three times. A representative result is presented.