Figure 3. HemS heme blotting.

After SDS gel electrophoresis, one gel was stained with comassie brilliant blue R. Another gel was transferred to a nitrocellulose filter to do heme blotting and detected by ECL. (A) Coomassie blue staining: Line 1, 5 µg BSA; Line 2, 4 µg HemS; (B) Heme binding: Line 1, 5 µg BSA; Line 2, 4 µg HemS. Experiment was performed in triplicate and a single representative experiment is presented.