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. 2012 May 31;7(5):e37640. doi: 10.1371/journal.pone.0037640

Figure 1. Comparing PCR equations.

Figure 1

In panel A, product formation (green circles) is modeled to accumulate with a perfect, constant efficiency of 100% (blue diamonds) using equation 4 (Information S1). The simulated data was fit using non-linear regression using the same function (black line). Panel B, simulated data of a purely reagent-limited reaction is shown using equation 5 with a maximum product yield of 5×106 (also fit to its function). Panel C, simulated data is shown using the PCR equation 6 with a max value of 5×106 and a Kd value of 5×105. The efficiency terms at each cycle were extracted and plotted as blue diamonds. Panel D shows examples of real qPCR data fitted to equation 6 from amplifications using cDNA libraries generated from total E. coli RNA as templates. The resulting fitting values were: rpsO, max  = 25.148, Kd = 1.6798, R2 = 0.99996; gapA, max  = 19.56, Kd = 1.5753, R2 = 0.99998; lacZ, max  = 16.29, Kd = 1.141, R2 = 0.99996.