Table 1. Human primer sequences used for RT–PCR.
| Gene | Accession | Sense primer | Antisense primer | Product size (bp) |
|---|---|---|---|---|
| SSEA-4 |
NM_006927 |
TGGACGGGCACAACTTCATC |
GGGCAGGTTCTTGGCACTCT |
118 |
| ABCG2 |
AY017168 |
AGTTCCATGGCACTGGCCATA |
TCAGGTAGGCAATTGTGAGG |
379 |
| ΔNp63 |
XM_036421 |
CAGACTCAATTTAGTGAG |
AGCTCATGGTTGGGGCAC |
440 |
| Pax 6 |
NM_00028 |
ATAACCTGCCTATGCAACCC |
GGAACTTGAACTGGAACTGAC |
208 |
| AE-5 |
NM_057808 |
CTACCTGGATAAGGTGCGAGCT |
TCTCGCATTGTCAATCTGCA |
150 |
| Keratocan |
NM_007035 |
ATCTGCAGCACCTTCACCTT |
CATTGGAATTGGTGGTTTGA |
167 |
| LPL |
XM_044682 |
GAGATTTCTCTGTATGGCACC |
CTGCAAATGAGACACTTTCTC |
276 |
| Osteocalcin |
X53698 |
ATGAGAGCCCTCACACTCCTC |
GCCGTAGAAGCGCCGATAGGC |
294 |
| Collagen II |
NM_001844 |
TTTCCCAGGTCAAGATGGTC |
CTTCAGCACCTGTCTCACCA |
377 |
| Aggrecan |
X17406 |
TGAGGAGGGCTGGAACAAGTACC |
GGAGGTGGTAATTGCAGGGAACA |
350 |
| GAPDH | M33197 | GCCAAGGTCATCCATGACAAC | GTCCACCACCCTGTTGCTGTA | 498 |
The table details the primers and sequences, their accession and expected product size after RT-PCR.