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Adult mouse lacrimal gland spheres were treated by trypsin/EDTA, and cultured for further epithelial characterization. All sphere-forming cells showed expression of epithelial cell markers, CK14 (A), confirmed by nuclear staining (D), and merged images (G). CK8 was partially positive in these cells (B), confirmed by nuclear staining (E), and merged images (H). However, no positive expression of α-SMA was observed (C, F, and I). Scale bars, 50 µm.
