Table 1. Primers and probes used for RT-qPCR assay.
| Gene symbol (accession number) | Primer and probe sequence (5′-3′) | Amplicon |
|---|---|---|
|
MMP-2 |
F: CTGGGAGCATGGAGATGGATA |
96 bp |
| (NM_008610) |
R: AAGTGAGAATCTCCCCCAACAC |
|
| |
P: FAM-ACATGCCTTTGCCCCGGGCA-TAMRA |
|
|
MMP-8 |
F: CAATTCCGGTCTTCGAGGAA |
85 bp |
| (NM_008611) |
R: TCCCAGTCTCTGCTAAGCTGAA |
|
| |
P: FAM-CCACGATGGTTGCAGAGAAGCTTAAAGA-TAMRA |
|
| MMP-9 |
F: GGGTCTAGGCCCAGAGGTAA |
86 bp |
| (NM_013599) |
R: AGACACGCCCCTTGCTGA |
|
| |
P: FAM-CCACGTCAGCGGGCTTCTCCC-TAMRA |
|
|
MMP-13 |
F: AAGTGTGACCCAGCCCTATC |
156 bp |
| (NM_008607) |
R: CACATGGTTGGGAAGTTCTG |
|
| |
P: FAM-CTTCTGGCGCCTGCACCCTC-TAMRA |
|
|
TIMP-1 |
F: GGACCTGGTCATAAGGGCTA |
175 bp |
| (NM_011593) |
R: GGCATATCCACAGAGGCTTT |
|
| |
P: FAM-TCTGCGGCATTTCCCACAGC-TAMRA |
|
|
TIMP-2 |
F: CTGTCCCATGATCCCTTGC |
87 bp |
| (NM_011594) |
R: TGGTGCCCATTGATGCTCT |
|
| |
P: FAM-CATCTCCTCCCCGGATGAGTGCC-TAMRA |
|
|
Col3a1 |
F: GGCAGTGATGGGCAACCT |
86 bp |
| (NM_009930) |
R: GGTCCAACTTCACCCTTAGCA |
|
| |
P: FAM-CCCCCTGGCCCTCCTGGAACT-TAMRA |
|
|
Col4a1 |
F: ATTAGCAGGTGTGCGGTTTG |
77 bp |
| (NM_009931) |
R: CACTGCGGAATCTGAATGGT |
|
| |
P: FAM-AGCACCGGCCATGGTGATGGC-TAMRA |
|
|
RPL-5 |
F: GGAAGCACATCATGGGTCAGA |
70 bp |
| (NM_016980) |
R: TACGCATCTTCATCTTCCTCCATT |
|
| P: FAM-TGTGGCAGACTACATGCGCTACC-TAMRA |