Fig. 1.

Identification of candidate nuclear GzmA substrates. K562 nuclei were treated with 1 μM GzmA or buffer for 30 min at 37 °C, and nuclear proteins were resolved using 2D gel electrophoresis and visualized by silver staining. Spots that changed in intensity at least 10-fold after GzmA treatment were grouped into 42 spots that migrated with similar apparent molecular weight and were analyzed by mass spectrometry. Forty-four GzmA candidate nuclear substrates (Table S1 and Dataset S1) were analyzed by Ingenuity software for known protein–protein interactions. Proteins with similar functions that are not annotated as interacting were added. Previously unknown targets are in blue, previously validated substrates that scored as hits are indicated in green, and those not scored as hits are in yellow.