Fig. 4.

2OHOA induces glioma cell differentiation in vitro and in vivo. Top, phase contrast micrographs of SF767 and U118 (human glioma) cells maintained in the presence or absence (control) of 2OHOA for 72 h (Left, 200 μM, 200x magnification). 2OHOA induced the expression of GFAP and GS (right) in SF767 cells as determined in immunoblots. Bottom, In vivo near infrared detection of a fluorolabeled anti-GS antibody in nude mice with SF767-derived tumors following treatment with the vehicle lone (control), 2OHOA and TMZ (Left, N = 10 per group) and the corresponding quantification (Right, GSNIR, mean ± SEM). GS was also determined ex vivo in these animals using immunoblotting (GSIB). GFAP was also quantified by immunocytochemistry (GFAPIC, Right).