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. 2012 May 14;109(22):8540–8545. doi: 10.1073/pnas.1204232109

Fig. 3.

Fig. 3.

Verification of the necessary role of TKase in the biosynthesis of the tetrahydroisoquinoline family. (A) HPLC analysis (UV at 270 nm). I, WT S. lusitanus NRRL 8034. II, mutant S. lusitanus TG3001 (ΔnapB); III, mutant S. lusitanus TG3003 (TG3001 harboring the napB–napC–napD expression plasmid pTG1012); IV, mutant S. lusitanus TG3005 (TG3001 harboring the qncN–qncM–qncL expression plasmid pTG1013). (B) LC-MS analysis. I, WT S. lusitanus NRRL 8034; II, mutant S. lusitanus TG3002 (ΔnapD); III, mutant S. lusitanus TG3004 (TG3002 harboring the napB–napC–napD expression plasmid pTG1012); IV, mutant S. lusitanus TG3006 (TG3002 harboring the qncN–qncM–qncL expression plasmid pTG1013). ▼, NDM.