Fig. 1.

The presence of an intron leads to increased accumulation of INO1 and ASC1 mRNA. (A and D) Schematic depiction of intron-containing INO1 and ASC1 genes indicating A1 and A2 primers used in RT-PCR analysis. (B and E) RT-PCR analysis of INO1 and ASC1 genes with and without an intron. The reverse transcription was performed by using oligo dT, and the cDNA obtained was PCR amplified by A1 and A2 primers. The control PCR represent the ACT1 RNA levels, which is used as a loading control indicating that equal amounts of template were present in each RT-PCR. (C and F) Quantification of transcript levels of the intron-less and intron-containing INO1 and ASC1.