Fig. 3.

Gene looping accompanies intron-mediated stimulation of transcription of INO1 and ASC1. (A and D) Schematic depiction of the intron-containing INO1 and ASC1 genes, indicating the position of restriction sites (vertical lines) and PCR primers used in CCC analysis. (B and E) CCC analysis of intronless and intron-containing INO1 and ASC1. The PCR products are indicated by the primers used for amplification. A P1-T1 PCR product indicates a looped configuration. The F1-R1 PCR represent the loading control indicating that equal amounts of template DNA were present in each CCC PCR. (C and F) Quantification of the CCC results shown in B and E.