Fig. 4.

Interaction of splice sites with the promoter and terminator regions during gene looping. (A and D) Schematic depiction of intron-containing INO1 and ASC1, indicating the position of restriction sites (vertical lines) and PCR primers used in the high-resolution CCC mapping. (B, C, E, and F) PCR products derived from the indicated primer pairs by CCC analysis show that the promoter interacts with the 5′ splice site and the terminator with the 3′ splice site in the intron-containing INO1 and ASC1. The CCC positive control PCR products were generated to check the amplification efficiency of the primer pairs in the PCR.