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. 2012 May 14;109(22):8528–8533. doi: 10.1073/pnas.1121116109

Fig. 2.

Fig. 2.

Single-particle EM analysis shows how FEN1 interacts flexibly with 9-1-1 and adopts a fixed position on 9Δ-1-1 in the presence of the DNA substrate. (A) Representative reference-free 2D class averages (top and side views) for the 9-1-1/FEN1 binary complex are compared with those corresponding to the 9Δ-1-1/FEN1/DNA ternary complex. Top and side views of the 9-1-1 complex are shown (Bottom) for reference. (B) Side and top views of the 9Δ-1-1/FEN1/DNA 3D reconstruction. (C) MDFF flexible fitting of the 9Δ-1-1/FEN1/DNA complex into the 3D map of the ternary complex.