Fig. 1.

Motor colocalization. (A) Immunofluorescence images show antibody staining of APP, KLC1, and DHC1 at 100×. (B, Upper) Detected APP features (vesicles) within an axon. Blue dots represent local maxima, red dots mark approximate (nearest pixel) position of subpixel Gaussian fits, and the overlap of blue and red is shown in pink. Gray contour denotes user-defined region of interest. (Lower) Enlargement of the three channels, corresponding to anti-APP (seed), anti-KLC1, and anti-DHC1 antibody staining. Red circles outline the 300-nm cutoff radius for colocalization and are centered at the precise subpixel coordinates of each APP Gaussian fitting. Arrowheads point to the position of two separate APP detected vesicles, showing association with KLC1 only (Right) or both KLC1 and DHC1 (Left).