Fig. 1.

Supplemented PUFAs are rapidly elongated, robustly incorporated into membranes, and released by PLA₂. RAW cells were incubated for 24 h in nonsupplemented media, or with 25 μM AA, EPA, or DHA. (A and B) The supplemented PUFAs were increased in membrane phospholipids in both their original forms and in their elongated forms. (C and D) After 2-h KLA (100 ng/mL) stimulation, supplemented and elongated PUFA species were increased in the media. Values in B and D represent area of the indicated fatty acid normalized to a deuterated DHA internal standard. PUFAs were analyzed with GC-MS and data are mean values of three biological replicates ± SEM.