Figure 5.

Rapid correction of hyponatremia induces dysregulation of gap junction proteins and myelin-related genes. (A) Quantification of for connexin genes expression by RT-PCR shows that astrocytic connexin 43 and oligodendroglial connexin 47 are substantially downregulated 12 hours postcorrection. Chronic hyponatremia did not cause any changes in connexin expression (P < 0.001 for both genes, ANOVA, n = 5 for all groups). (B) Downregulation of CNPase gene expression was observed during hyponatremia and at 12 hours postcorrection, but we did not observe changes in myelin basic protein (MBP) mRNA levels in either conditions (n = 5 for each group, * P < 0.001 by ANOVA for CNPase and NS for MBP). Cx, connexin; MBP, myelin basic protein; CNPase, 2′,3′-cyclic nucleotide 3′-phosphodiesterase.