Fig. 1.

Agarose gel (1.5%) stained with ethidium bromide showing amplicons of the expected size (463 bp) from RT-PCR reactions with nucleic acids extracted from the equivalent of 1/4 viruliferous adult female Paratrichodorus allius (lane 4-6), reared in the greenhouse. Lane 7 is TRV isolated from a symptomatic potato tuber cv. Ranger Russet as the positive control. Lane 1 is water; lane 2 is cDNA from Caenorhabditis elegans as negative control; and lane 3 in cDNA from non-viruliferous P. allius from spearmint plants, as negative control. Lane (M) is a 100 bp DNA ladder (Invitrogen).