Fig. 1.

Method for collecting early A. besseyi embryos. (A) A. besseyi is cultured on a fungal mat of B. cinerea grown on PDA for a few days at 30°C. (B) After a few days of culturing, the nematodes are reproducing and some of them have moved from the surface of the PDA to the lid of the Petri dish. Nematodes in web-like aggregations are visible (inset of B). (C) Nematodes are collected from the lid with sterile water, transferred to a new fungal mat of B. cinerea grown on 1/10 PDA, and incubated for 24 to 48 hours at 30°C. (D) After the incubation, many eggs are laid on the PDA plate. Many early embryos are collected and transferred to an 8-well slide (inset of D). Scale bar, B, 1 mm; D, 200 μm.