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AQP2 is recovered in Triton X-100-insoluble fraction. Samples of Triton X-100-soluble (S) and -insoluble (I) fractions from MDCK-hAQP2 cell lysates with (+) or without (–) forskolin treatment for 30 min were subjected to SDS-PAGE and immunoblotting using anti-AQP2 and anti-caveolin-1 (Cav1) antibodies.
