Fig. 4.

AQP2 co-immunoprecipitates with caveolin-1. a: In immunoprecipitates with anti-AQP2, caveolin-1 is detected as a dense band in forskolin-treated cells (F) and a faint band in control cells (C) at 22–24 kDa (arrow) with anti-caveolin-1 (Cav1). Dense bands both around 20 kDa (arrowhead) and 40 kDa (double-arrowhead) seem to be non-specific because these bands are also detected in the lane in which rabbit IgG was electrophoresed (RbIgG). b: Immunoprecipitates with anti-AQP2 from each cell lysate described as follows were subjected to immunoblotting with anti-caveolin-1 (Cav1). –: control cells, +: cells treated with forskolin for 30 min, ±: cells treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min.