FIG. 2.

Contribution of different NOS isoforms to basal NO metabolite concentrations in plasma and tissues of mice. All animals were maintained on a standard rodent chow; wild-type controls (C57BL/6J) are represented by white bars (n=6–8) and isoform-specific knockouts by shaded bars (n=4–9). For illustrative purposes, all protein-bound NO moieties (including S- and N-nitroso and NO-heme species) are displayed as total nitrosylation products (the higher-resolution picture detailing individual changes in either NO species can be found in Supplementary Fig. 1 online). Left-hand panels show steady-state concentrations in individual compartments, right-hand panels display a theoretical total body concentration for each NO metabolite pool as a weighted average. Data are mean±SEM, *p<0.05, ** or ^#p<0.01.