FIG. 10.

Differential induction of Nox isoforms by Ang II and effects of inhibitors of AT₁R-PLC-PKC-Nox pathway on Ang II-induced changes in ROS and CYP11B2 activity in rat adrenal cortical slices. The changes in Nox1, Nox2, and Nox4 mRNA expression (A) are shown. Comparison of the effects of antioxidants (NAC, 200 μM and MnTMPyP, 100 μM), H₂O₂ scavenger (PEG-Cat, 350 U/ml), inhibitor of Nox (VAS-2870, 20 μM), NOS (L-NAME, 10 μM), PLC (U73122, 10 μM) and PKC (BIS, 10 μM), and AT₁R antagonist (losartan, 10 μM) on Ang II (100 nM for 6 h)-induced changes in MDA levels (B) and CYP11B2 activity (C) are shown. Results derived from six independent experiments are presented as mean±SEM. ** and ^##p<0.01, and n.s., not significant. ** denotes a significant difference between the control and Ang II treated adrenal cortical slices. ## denotes a significant difference between Ang II and Ang II+drug-treated adrenal cortical slices. PKC, protein kinase C; BIS, bisindolylmaleimide.