FIG. 9.

Ang II-induced changes in CYP11B2 activity and mRNA expression and ROS levels in rat adrenal cortex. Rat adrenal cortical slices treated with increasing concentrations of Ang II (10, 100, and 1000 nM for 6 h) were analyzed for CYP11B2 activity (A), CYP11B2 mRNA (B), and MDA levels as assessed by T-BARS assay (C), protein carbonyl levels (D), superoxide formation (E), and H₂O₂ levels (F). Slices not treated with Ang II served as CON. Superoxide and H₂O₂ generation was measured spectrophotometrically by monitoring SOD (2000 U/ml)-inhibitable, NADPH-dependent oxidation of cytochrome C (80 μM) and catalase-inhibitable, oxidation of Amplex Red (100 μM) to resorufin, respectively, as described in Materials and Methods section. Results derived from six independent experiments are presented as mean±SEM. ** and ^##p<0.01. ** denotes a significant difference between the control and Ang II treated adrenal cortical slices. ## denotes a significant difference between Ang II and Ang II+drug-treated adrenal cortical slices. MDA, malondialdehyde.