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P38 and protein carbonyls in HT22 cells. HT22 cells were subjected to two cycles of 24 h of ethanol (50 or 100 mM) exposure + 4 h of ethanol washout. P38 inhibitor SB203580 (200 nM) or vehicle was added at the beginning of the first ethanol washout period; 4 h later, mitochondria were isolated to measure protein carbonyl content. Values are means ± SE of 4 experiments/group. *Significant difference vs. 0 mM ethanol (P < 0.05). †Significant difference vs. vehicle (P < 0.001).
