Figure 4.

Rescue of Sua7 mislocalization by WT and mutant KAP114. The Δkap114 Δulp2 double mutant carrying the plasmid YCpGAL–GST–GFP–SUA7 was transformed with empty vector, pRS315–KAP114, or pRS315–KAP114 K909R. The cells were cultured in liquid medium containing raffinose, and synthesis of the GFP–Sua7 fusion protein was induced by the addition of 2% galactose. After 2 h, the strains were analysed by direct fluorescence microscopy. The number of cells showing a predominantly nuclear versus a cytoplasmic localization of the GST–GFP–Sua7 fusion protein was quantified. Data shown in the bar graphs represent the average values±standard deviations of three independent experiments. Scale bar, 5 μm.