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. 2012 May 11;31(11):2604–2614. doi: 10.1038/emboj.2012.139

Figure 2.

Figure 2

14-3-3–MKLP1 and ARF6–MKLP1 interactions are mutually exclusive. (A) Effect of excess ARF6 on the interaction between MKLP1 and 14-3-3 in the crude extract from mitotic HeLa cells treated with an Aurora inhibitor ZM447439, which promotes the MKLP1–14-3-3 interaction. A constitutively active (locked in GTP-form) ARF6 Q67L, but not a GTP-binding deficient T27N mutant, interfered with the MKLP1–14-3-3 interaction. (B) Repression of the ARF6–MKLP1 interaction by excess 14-3-3 in the extracts from mitotic HeLa cells treated with or without ZM447439 (+ZM and −ZM, respectively). Inhibition of Aurora kinase enhanced the interference by 14-3-3.