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. 2012 May 11;31(11):2604–2614. doi: 10.1038/emboj.2012.139

Figure 4.

Figure 4

Defects in the MKLP1–ARF6 interaction cause cytokinesis failure. (A) Schematic of the protocol for knockdown and rescue experiments. (B) Cells were synchronised and transfected with MKLP1 or control siRNA and lysed directly into SDS-loading buffer. Samples were separated using SDS–PAGE and probed for MKLP1 with anti-MKLP1 antibody by western blotting. (C) Cells were transiently transfected with GFP–MKLP1 variants as indicated. Whole cell lysate was probed for GFP–MKLP1 with anti-GFP antibody. (D) Quantitation of cytokinesis failures from live cell recordings of HeLa cells treated with MKLP1 siRNA and transfected with GFP vector or the MKLP1 variants as indicated. The graph shows the percentage of cells that failed cytokinesis averaged from three independent experiments in which at least 100 GFP–MKLP1 or GFP expressing cells were analysed. Error bars indicate the standard deviation. (E) The data of the cytokinesis failure were analysed by generalised linear model for binomial data and P-values after the Tukey correction for multiple comparison are shown.