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. 2012 May 11;31(11):2604–2614. doi: 10.1038/emboj.2012.139

Figure 6.

Figure 6

Defects in the MKLP1–ARF6 interaction disintegrate the midbody. (A) Stills from the knockdown and rescue experiments with the GFP–MKLP1-V786A mutant performed in the HeLa cells expressing mCherry–tubulin. (B, C) Stills from the knockdown and rescue experiments with the mCherry–MKLP1-V786A mutant performed in the HeLa cells expressing GFP–tubulin and GFP–CENP-A (B) or GFP–CEP55 (C). Arrowheads in (B) indicate the intact midbody remnant with distinct signal for both MKLP1 and tubulin. Arrows indicate the residual MKLP1 signal in the regressing cell lacking a distinct tubulin signal. Arrowheads in (C) indicate the fragments from the disintegrating Flemming body. Bar, 5 μm.