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. 2012 Apr 17;31(11):2486–2497. doi: 10.1038/emboj.2012.91

Figure 3.

Figure 3

Expression analysis of conditional Nrbp1 mice. (A) qRT–PCR analysis of the WNT target genes, Cd44, c-Myc and Ccnd1 in the intestine of cKO and C mice at 4–5 days post tamoxifen administration. (B) Immunohistochemical (i, ii) and in situ analysis (iii, iv) of small intestines from C and cKO mice at 7–9 days post tamoxifen treatment indicating loss of Nrbp1 causes an increase in (i) c-Myc ( × 400 magnification), (ii) Sox9 ( × 400 magnification), (iii) Mmp7 ( × 400 magnification) and (iv) Lgr5 ( × 200 magnification). (C) Immunohistochemical analysis of small intestines from C and cKO mice at 7–9 days post tamoxifen treatment show increased expression of (i) pErk ( × 200 magnification), but no obvious increase in (ii) nuclear Ctnnb1 signal ( × 400 magnification). *P<0.05, **P<0.01.