Skip to main content
. 2012 Apr 24;31(11):2615–2628. doi: 10.1038/emboj.2012.93

Figure 4.

Figure 4

Caspase-2 is required for aerolysin-mediated apoptosis. (A) Representative dot plots of flow cytometric analysis of control and caspase-2-knockdown cells upon aerolysin toxin treatment (5 ng/ml for 48 h). Cells were subjected to annexin-V and PI staining and the percentage of cells displaying annexin-V single positivity (Q4) and annexin-V, PI double positivity (Q2) are denoted. (B) Western blot analysis of HeLa cells treated with aerolysin for 24 h. The processing of caspase-2 and PARP was monitored (FL—full-length protein, *—processed form). (C) Flow cytometric analysis of caspase-2-knockdown HeLa cells 48 h after aerolysin (5 ng/ml) treatment and (D) Caspase-2-/- MEFs 24 h after aerolysin (5 ng/ml) treatment. Percentage of dead cells represents the percentage of cells displaying both annexin-V single positivity and annexin-V, PI double positivity out of the total number of cells (*=P<0.05, **=P<0.01, ***=P<0.005). Figure source data can be found with the Supplementary data.