Figure 4.

T cell development in MHC class I- and class II-restricted TCR transgenic mice in the absence of MAZR (a) CD4 and CD8 expression pattern on thymocytes and lymph node T cells isolated from Mazr^+/+,OT-II and Mazr^−/−,OT-II littermates. Numbers indicate the percentage of cells in the respective quadrants. Data are representative of 3 independent experiments. (b) Histograms show V_α2 expression on CD4⁺ and CD8⁺ lymph node T cells isolated from Mazr^+/+,OT-II and Mazr^−/−,OT-II littermates. Numbers show the percentage of V_α2^hi cells within the indicated region. Gating areas for CD4⁺ and CD8⁺ T cell populations are shown in the lower panel of a. Data are representative of 3 independent experiments. (c) CD4 and CD8α expression on total (upper panel) and CD3^hi (middle panel) thymocytes, and on lymph node T cells (lower panel) isolated from Mazr^+/+,OT-I and Mazr^−/−,OT-I littermates. Numbers indicate the percentage of cells in the respective quadrant. Data are representative of 7 independent experiments. (d) Histograms show V_α2 expression on CD4⁺ and CD8⁺ lymph node T cells isolated from Mazr^+/+,OT-I and Mazr^−/−,OT-I littermates. Numbers show the percentage of V_α2^hi cells within the indicated region. Gating areas for CD4⁺ and CD8⁺ T cell populations are shown in the lower panel of c. Data shown are representative of 7 independent mice. (e) Panels showing the percentage of V_α2^hi cells in CD4⁺ lymph node T cells isolated from Mazr^+/+,OT-I and Mazr^−/−,OT-I mice. Each symbol represents one mouse, and horizontal lines indicate average values.