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. Author manuscript; available in PMC: 2013 May 8.
Published in final edited form as: Biochemistry. 2012 Apr 26;51(18):3786–3798. doi: 10.1021/bi300147m

Figure 7.

Figure 7

(a). Paramagnetic broadening of P1 amide resonances by CheY mutants spin-labeled with MTSL. 15N-labeled P1P2 was mixed with CheY constructs labeled with MTSL at various positions, including E89C used in the experiment shown here. The ratio of P1 amide intensity with the oxidized- (active) and the reduced (inactive) spin-label was plotted across the P1 sequence. An arbitrary intensity ratio of 1.40 was assigned to residues for which the intensity ratio could not be calculated due to resonance overlap or lack of assignment. Assignments of the distance restraints, as well as the results obtained with CheY constructs containing the spin-labels at other positions are summarized in Table 2.