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. Author manuscript; available in PMC: 2013 May 8.

Published in final edited form as: Biochemistry. 2012 Apr 26;51(18):3786–3798. doi: 10.1021/bi300147m

Table 2.

Summary of the observed intermolecular NOE^a and long-range distance constraints^b obtained from MTSL experiments

^aCheY nuclei	P1 nuclei
T16 H_γ2^#	T11 HN, F12 HN, F13 HN, D14 HN, E15 HN, A16 HN
R18 HN	T11 H_γ2^#
R19 H_β^#	T11 H_γ2^#, E15 HN
R19 HN	T11 H_γ2^#
120 Hδ1^#	M3 H_ε^#, F8 HN, T11 H_γ2^#, F12 HN, T56 H_γ2^#
120 HN	T11 H_γ2^#
N23 Hδ^#	T11 H_γ2^#
N23 HN	M3 H_ε^#, T11 H_γ2^#
L24 HN	M3 H_ε^#
K109 HN	M3 H_ε^#
F111 HN	M3 H_β1, M3 H_γ1, M3 H_ε^#, 15 H_γ2^#, 15 Hδ^#

^bMTSL position on CheY	P1 residues broadened by MTSL
E89C	52HN, 53HN, 55HN, 58HN, 60–66HN, 68HN
K91C	Q63 H_ε^#, N71 Hδ^#

^b

only the results used in the structure calcuation are shown here

^#

indicates the three equivalent protons on methyl groups