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. 2012 May 28;197(5):595–604. doi: 10.1083/jcb.201110047

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© 2012 Gebert et al.

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Figure 4. — Mgr2 promotes Δψ-dependent protein import. (A) b₂(84)-DHFR was imported into wild-type (WT), tim21Δ, and mgr2Δ mitochondria (cells grown at 30°C) in the presence of CCCP and analyzed by SDS-PAGE and autoradiography. Import without CCCP was set to 100% (control). The mean ± SEM (n = 4) is shown. (B and C) Preprotein import into mitochondria followed by SDS-PAGE. MTX, methotrexate; p, precursor; i, intermediate; m, mature.